Laboratory of Pathophysiology

Development of Research Techniques

 

• Ultra-rapid 1D& 2D confocal Ca2+ imaging (≥ 240 Hz) [Nikon A1 system]

• Spark/image analysis programming [“Pic” software written in C++]

• Automatic application of pressurized fluid

• Adenoviral KD of IP3R

• IP3R over-expression

• Culture of isolated adult cardiac cells

• TAC model

• ZP preparation

 

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Basic Techniques

 

• Adult cardiac cell isolation

• Patch clamp

• Immunocytochemistry

• Western blotting, PCR

• Organelle staining/visualization

• Monitoring of single cell contraction

• 3D confocal imaging